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目的 从人肠道菌群中筛选并鉴定能够将芒果苷转化为芒果苷元的菌株。方法 将人肠道菌群与芒果苷在厌氧条件下共孵育,通过高效液相色谱法(HPLC)检测转化产物,分离纯化具有转化活性的菌株,并对其进行鉴定及转化特性研究。结果 从6份人肠道菌群样本中筛选出1份能转化芒果苷的样本,并从中分离得到1株具有转化能力的单菌株N-9,经鉴定为鸟肠球菌(Enterococcus avium)。转化实验显示,该菌株可在6h内将芒果苷完全转化为芒果苷元,并能转化异荭草素和异牡荆素两种黄酮碳苷。结论 本研究筛选获得1株可转化芒果苷的人源肠道菌株,并对其基本特性进行研究,可为芒果苷的生物转化研究提供实验参考。
Abstract：Objective To screen and identify strains from the human gut microbiota capable of converting mangiferin to norathyriol.Methods Human gut microbiota was anaerobically co-incubated with mangiferin.Conversion products were detected with high performance liquid chromatography.Strains exhibiting conversion activity were isolated and purified,and their conversion characteristics were identified.Results One out of the six human gut microbiota samples was selected,which was capable of converting mangiferin.A single convertible strain,N-9,was isolated from this sample and identified as Enterococcus avium.The conversion experiments demonstrated that this strain completely converted mangiferin to norathyriol within 6 h.Furthermore,the strain might also convert into two flavonoid glycosides:isoorientin and isovitexin.Conclusion This study identifies a human gut bacterial strain capable of converting mangiferin.Its fundamental properties were characterized,providing an experimental reference for mangiferin biotransformation.
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基本信息:
中图分类号:R285
引用信息:
[1]聂宇迪,吴玥,杨澜,等.可转化芒果苷的人源肠道菌株的筛选鉴定及转化特性研究[J].中南药学,2026,24(06):1-7.
基金信息:
国家自然科学基金面上项目(No.82374134); 北京中医药大学“揭榜挂帅”项目(No.2022-JYB-JBZR-021)
2026-05-08
2026-05-08
2026-05-08