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目的 分析参芪复脉颗粒化学成分及入血成分，阐明其可能的药效物质基础。方法 采用UPLC-Q-TOF-MS技术和Peakview 2.0软件对参芪复脉颗粒进行数据采集和处理，结合保留时间、相对分子质量、二级质谱碎片以及对照品二级质谱库分析鉴定参芪复脉颗粒的体内外成分。结果 从参芪复脉颗粒中共鉴定出118个化学成分，其中包括木脂素类19个、黄酮类27个、有机酸类8个、三萜皂苷类29个、甾体皂苷7个、氨基酸类2个、脂肪酸类4个、生物碱2个、三萜类3个、维生素1个、三萜酸类1个、脂肪醇1个、其他类14个，从灌胃后的小鼠血清中推测出33个入血原形成分。结论 本研究运用UPLC-Q-TOF-MS技术及中药血清药物化学方法，分析了参芪复脉颗粒的化学成分及入血成分，初步探究了其可能的药效物质基础，可为参芪复脉颗粒的进一步开发利用提供参考依据。
Abstract：Objective To analyze the chemical components and blood components in Shenqi Fumai granules,and to elucidate their potential pharmacological material basis.Methods UPLC-Q-TOF-MS technology and Peakview 2.0 software were used for the data collection and processing of Shenqi Fumai granules.The components were identified via retention time,relative molecular mass,secondary mass spectrometry fragments,and secondary mass spectrometry library of reference substances.Results Totally 118 chemical components were identified from Shenqi Fumai granules,including 19 lignans,27 flavonoids,8 organic acids,29 triterpenoid saponins,7 steroidal saponins,2 amino acids,4 fatty acids,2 alkaloids,3 triterpenoids,1 vitamin,1 triterpene acid,1 fatty alcohol and 14 other compounds.Totally 33 prototype blood components were inferred from the serum of mice after gavage.Conclusion UPLC-Q-TOF-MS technology and serum pharmacochemistry of traditional Chinese medicine are used to analyze the chemical components and blood components of Shenqi Fumai granules,and preliminarily explore their potential pharmacological material basis,providing a reference for the further development and utilization of Shenqi Fumai granules.
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基本信息:
DOI：
中图分类号:R286.0;O657.63
引用信息:
[1]李安祁,霍金海,李秀伟,等.基于UPLC-Q-TOF-MS技术的参芪复脉颗粒体内外成分研究[J].中南药学,2025,23(10):2803-2813.
基金信息:
国家中药材产业体系(No.CARS-21); 黑龙江省“头雁”团队支持资金(中医药防治慢病创新研究及产品开发团队)