中南药学

目的 优化超声辅助复合酶法提取赤芍多糖的工艺，检测其体外抗氧化活性。方法 考察酶解时间、液料比、酶解温度、pH值、复合酶添加量对赤芍多糖提取率的影响，采用响应面法进行提取条件优化并对赤芍多糖清除自由基的能力进行研究。结果 超声辅助复合酶提取赤芍多糖最佳提取工艺为：酶解时间60 min、液料比30∶1(mL/g)、酶解温度61℃、pH 4.6、复合酶添加量2%，酶解后，在超声功率500 W、温度55℃下超声45 min，此条件下，实际提取率为22.87%。赤芍多糖质量浓度为8 mg/mL时，赤芍多糖清除DPPH自由基、超氧阴离子自由基及羟基自由基能力达到维生素C组的78.07%、61.17%、22.97%。结论 本研究通过超声辅助复合酶法优化了赤芍多糖提取工艺并证明赤芍多糖具有抗氧化能力。

Objective To optimize the extraction of polysaccharides from Paeonia lactiflora by an ultrasound-assisted multi-enzyme method and to evaluate their in vitro antioxidant activity.Methods The effects of enzymatic hydrolysis time,liquid-to-solid ratio,enzymatic hydrolysis temperature,pH,and multi-enzyme dosage on the polysaccharide yield were determined.Response surface methodology was used to optimize the extraction conditions.Additionally,the radical scavenging abilities of the extracted polysaccharides were assessed.Results The optimal extraction conditions were as follows:ultrasonic treatment for 45 min,ultrasonic temperature at 55℃,enzymatic hydrolysis for 60 min,liquid-to-solid ratio at 30∶1 (mL/g),enzymatic hydrolysis temperature at 61℃,pH 4.6,and 2% multi-enzyme addition under specified ultrasonic power.Following enzymatic hydrolysis,the mixture was subjected to ultrasonic treatment for 45 min with parameters set at 500 W power,and 55℃.Under these conditions,the actual extraction yield of polysaccharides reached 22.87%.At 8 mg/mL,the scavenging rates for DPPH,superoxide anion radical,and hydroxyl radicals were 78.07%,61.17%,and 22.97%,respectively,relative to those of the vitamin C group.Conclusion This method effectively optimizes the extraction of Paeonia lactiflora polysaccharides with in vitro antioxidant activity.