绿色荧光蛋白在药学研究中的应用
张敏,任慧霞
摘要(Abstract)：
关键词(KeyWords)： 绿色荧光蛋白;药物;研究
基金项目(Foundation):
作者(Author): 张敏,任慧霞
参考文献(References)：
- [1]Misteli T,Spector DL.Applications of the green fluorescentprotein in cell biology and biotechnology[J].Nat Biotechnol,1997,15(10):961-964.
- [2]Dickson RM,Cubitt AB,Tsien RY,et al.On/off blinking andswitching behaviour of single molecules of green fluorescent pro-tein[J].Nature,1997,388(6640):355-358.
- [3]Yang TT,Sinai P,Green G,et al.Improved fluorescence anddual color detection with enhanced blue and green variants of thegreen fluorescent protein[J].Biol Chem,1998,273(14):8212-8216.
- [4]Gonzalez JE,Negulescu PA.Intracellular detection assays forhigh-throughput screening[J].Curr Opin in Biotechnol,1998,9(6):624-631.
- [5]Schmid JA,Birbach A,Hofer-Warbinek R,et al.Dynamics ofNF kappa B and Ikappa Balpha studied with green fluorescentprotein(GFP)fusion proteins.Investigation of GFP-p65 bind-ing to DNa by fluorescence resonance energy transfer[J].J BiolChem,2000,275(22):17035-17042.
- [6]Horgan AM,Stork PJ.Examining the mechanism of Erk nucle-ar translocation using green fluoscent protein[J].Exp CellRes,2003,285(2):208-220.
- [7]Scott ES,Nalcomber S,O Hare P.Nuclear translocation andactivation of the transcription factor NFAT is blocked by herpessimplex virus infection[J].J Virol,2003,75(20):9955-9965.
- [8]Koster M,Hauser H.Dynamic redistribution of STAT1 proteinin IFN signaling visualized by GFP fusion proteins[J].Eur JBiochem,1999,260(1):137-144.
- [9]Kain SR.Green fluorescent protein(GFP):applications in cell-based assays for drug discovery[J].Drug Discov Today,1999,4(7):304-312.
- [10]Bevan N,Rees S.Pharmaceutical applications of GFP andRCFP[J].Methods Biochem Anal,2006,47(53):361-389.
- [11]Milligan G,Eds.Signal Transduction:A Practical Approach(A).Rees S,Brown S,Stables J.Reporter gene systems forthe study of G protein-coupled receptor signal transduction inmammalian cells[M].Oxford University Press,New York.,1999,2nd Ed,171-222.
- [12]Terstappen GC,Giacometti A,Ballini E,et al.Developmentof a functional reporter gene HTS assay for the identification ofmGluR7 modulators[J].J.Biomol.Scr,2000,5(4):255-262.
- [13]Subbaramaiah K,Bulic P,Lin Y,et al.Development and useof a gene promoter-based screen to identify novel inhibitors ofcyclooxygenase-2 transcription[J].J Biomol Scr,2001,6(2):101-110.
- [14]Nagy SR,Liu G,Lam KS,et al.Identification of novel Ahreceptor agonists using a high-throughput green fluorescentprotein-based recombinant cell bioassay[J].Biochemistry,2002,41(3):861-868.
- [15]陆锦玲,李鹏程,朱丹,等.利用非侵入性光学成像技术监测在体肿瘤的生长[J].激光生物学报,2003,12(4):282-287.
- [16]Boute N,Jockers R,Issad T.The use of resonance energytransfer in high-throughput screening:BRET versus FRET[J].Trends Pharmacol.Sci,2002,23(8):351-354.
- [17]Matayoshi ED,Wang GT,Krafft GA,et al.Novel f luorogen-ic substratesfor assaying retroviral proteases by resonance en-ergy transfer[J].Science,1990,247(4945):954-958.
- [18]Lindstren K,Menendez-Benito V,Masucci MG,et al.Atransgenic mouse model of the ubiquitin/proteasome system[J].Nat Biotechnol,2003,21(8):897-902.
- [19]岳莉莉,齐义鹏,邓岩卉,等.乙型肝炎病毒e抗原基因与绿色荧光蛋白基因的融合及其表达[J].中国病毒学,1997,12(4):336-341.
- [20]李正赫,田旺,杨文博,等.绿色荧光蛋白干扰素-α2b融合基因的构建与表达[J].中华微生物学和免疫学杂志,2005,25(7):582-582.
- [21]Mclean AJ,Bevan N,Rees S,et al.Visualizing differences inligand regulation of wild-type and constitutively active mutantbeta(2)-adrenoceptor-green fluorescent protein fusion pro-teins[J].Immunol Methods,1999,56(6):1182-1191.
- [22]Conway BR,Minor LK,Xu JZ,et al.Quantification of G-Pro-tein Coupled Receptor Internatilization Using G-Protein Cou-pled Receptor-Green Fluorescent Protein Conjugates with theArrayScantrade mark High-Content Screening System[J].JBiomol Screen,1999,4(2):75-86.
- [23]Hadjantonakis AK,Nagy A.The color of mice:in the light ofGFP-variant reporters[J].Histochem Cell Biol,2001,115(1):49-58.
- [24]David HE,Dawe AS,de Pmoerai DL,et al.Construction andevaluation of transgenic hsp16-GFP-lacZ Caenorhabditis ele-gans strain for environmental monitoring[J].Environ ToxicolChem,2003,22(1):111-118.
- [25]Liu H,Kishi T,Roseberry AG,et al.Transgenic mice ex-pressing green fluorescent protein under the control of the mel-anocortin-4 receptor promoter[J].Neuroscience,2003,23(18):7143-7154.
- [26]Hara M,Wang X,Kawamura T,et al.Bell GI.Transgenicmice with green fluorescent protein-labeled pancreatic beta-cells[J].Am J Physiol Endocrinol Metab.2003,284(1):177-183.
- [27]周旭,全东琴,崔光华.基因壳聚糖纳米粒表面修饰和转染研究[J].华北国防医药,2005,17(1):3-5.
- [28]马百坤,徐东刚,王嘉玺,等.血管生成素与绿色荧光蛋白融合基因的构建、表达及生物活性[J].中国生物化学与分子生物学报,2002,18(4):445-449.